[Array Studio Video Tutorial] DNA-Seq Analysis Basics: Getting Started With DNA-Seq Pipeline Analysis And Data QC
Omicsoft Next Generation Sequencing (NGS) analysis includes NGS (next generation sequencing) bioinformatics tools for the entire process, from QC to alignment to post-alignment summarizations and analysis. Array Studio provides a suite of tools to quickly, easily, and reliably process DNA-seq data. In this article, we introduce our tutorial on DNA-Seq analysis pipeline and data QC. We will discuss more on downstream analysis functions in the coming blog(s).
In Array Studio, users have the choice of either executing each step of the analysis one-by-one, or can use the DNA-seq pipeline function. Our video tutorial will walk you through the functions automatically executed by the standard DNA-seq pipeline, starting with raw reads in .fastq format.
If you choose to perform analysis step by step, before aligning your DNA-seq data, you must first perform quality control (QC) on the raw data, to spot common problems like adapter or barcode sequence contamination, degraded quality at ends of reads, or problematic samples. The Array Studio Raw Data QC Wizard reports a number of useful measures of raw NGS quality.
Additional information about how to interpret these functions can be found in the RNA-seq Raw Data QC Analysis video.
Additional aligned data QC metrics include:
1 Alignment Metrics
2 Flag Metrics
3 Profile Metrics
4 Insert Size Metrics
5 Duplication Metrics
6 Strand Metrics
Please check out our video tutorial Getting Started with DNA-seq pipeline functions for more detailed illustration.